The Abcam Human TSH ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TSH in serum, plasma, and cell culture supernatants.
This assay uses an antibody specific for human TSH coated on a 96-well plate. Standards and samples are pipetted into the wells and TSH present in a sample is bound to the wells by the immobilized antibody. The wells are washed and the biotinylated anti-human TSH antibody is added.
After washing unbound biotinylated antibodies, HRP-conjugated streptavidin is pipetted into the wells. The wells are washed again, a TMB substrate solution is added to the wells and the color develops in proportion to the amount of TSH bound. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm.
Key features and details
- Sensitivity: 4 pg / ml
- Range: 2,744 pg / ml – 2000 pg / ml
- Sample type: cell culture supernatant, plasma, serum
- Detection method: colorimetric
- Test type: Sandwich (quantitative)
- Reacts with: Human
Notes: Optimization with urine samples may be required.